Complete nucleotide sequence of the intergenic spacer between 25S and 17S rDNA in Miscanthus sinensis var. glaber
نویسندگان
چکیده
The nucleotide sequence of the intergenic spacer (IGS) between the 25S and 17S ribosomal RNA genes of Miscanthus sinensis var. glaber was determined. The spacer length (2,178 bp) in Miscanthus is close to that of rice IGS (2,140 bp). Many repeated elements with consensus lengths of 34 and 59 base pairs are found in the nontranscribed region (NTS), upstream of the putative transcription initiation site (TIS). Subrepeats B with four copies are longer and better conserved than subrepeats A with ten copies. The sequences of the external transcribed region (ETS) show high identity, ranging from 66% to 76%, among Miscanthus, rice, maize, and wheat.
منابع مشابه
Diversity and Inheritance of Intergenic Spacer Sequences of 45S Ribosomal DNA among Accessions of Brassica oleracea L. var. capitata
Ribosomal DNA (rDNA) of plants is present in high copy number and shows variation between and within species in the length of the intergenic spacer (IGS). The 45S rDNA of flowering plants includes the 5.8S, 18S and 25S rDNA genes, the internal transcribed spacer (ITS1 and ITS2), and the intergenic spacer 45S-IGS (25S-18S). This study identified six different types of 45S-IGS, A to F, which at 3...
متن کاملMolecular Organization of the 25S–18S rDNA IGS of Fagus sylvatica and Quercus suber: A Comparative Analysis
The 35S ribosomal DNA (rDNA) units, repeated in tandem at one or more chromosomal loci, are separated by an intergenic spacer (IGS) containing functional elements involved in the regulation of transcription of downstream rRNA genes. In the present work, we have compared the IGS molecular organizations in two divergent species of Fagaceae, Fagus sylvatica and Quercus suber, aiming to comprehend ...
متن کاملNucleotide sequence analysis of the Second Internal Transcribed Spacer (ITS2) in Hyalomma anatolicum anatolicum in Iran
Ticks are important acarina that infest animals. They are obligatory blood sucker arthropods which economically impact cattle industry by reducing weight gain and production. Moreover, they are important vectors of viral, bacterial, rickettsial and parasitic pathogens infecting humans and animals. In view of the importance of Hyalomma anatolicum anatolicum in pathogen transmission, including Th...
متن کاملThe Molecular Differentiation of Renibacterjum Salmoninarumisolates
The nucleotide sequences of the rRNA genes and the 5' flanking region were determined for R. salmoninarumATCC 33209T from overlapping products generated by PCR amplification from the genomic DNA. Comparisonof the sequences with rRNA genes from a variety of bacteria demonstrated the close relatedness between R.salmoninarum and the high G+C group of the actinobacteria, in particul...
متن کاملNucleotide sequence of the intergenic spacer and the 18S ribosomal RNA gene from mustard (Sinapis alba).
From a rRNA repeating unit (9.1 kbp) of mustard we have The DNA was sequenced according to the method of Maxam sequenced the first internal transcribed spacer, the 5.8S rRNA and Gilbert gene, and the second internal transcribed spacer [1]. Here, we present the nucleotide sequence of a 5500 bp fragment containing the 3 ' end terminus of the 25S gene (486 bp), the intergenic spacer REFERENCES (32...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 1998